Analysis Note

Growth promotion test according to ISO 11133.Inoculum on reference medium - Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100 - Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100 - Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100Recovery on test medium - Listeria monocytogenes ATCC 13932 (WDCM 00021): ≥ 70 % - Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 % - Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %Colonies - Listeria monocytogenes ATCC 13932 (WDCM 00021): blue-green; non-transparent zone - Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone - Listeria innocua ATCC 33090 (WDCM 00017): blue-greenGrowth - Escherichia coli ATCC 25922 (WDCM 00013): no growth - Escherichia coli ATCC 8739 (WDCM 00012): no growth - Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth - Enterococcus faecalis ATCC 29212 (WDCM 00087): no growthIncubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

Application

ChromoCult^® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult^® Listeria Selective Agar for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

General description

ChromoCult^® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L-A-phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 1004390017

Packaging

10 vial in Fibre case